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Development of Mammalian Stable Cell Line with HAC vectors

Human Artificial Chromosome (HAC) is an alternative vector that promise to overcome problematic transgene expression often occurring with conventional vectors in mammalian cells and bodies.

Chromo Research provides custom service in generation of mammalian stable cell lines with HAC technology for our customers' gene and cell of interest.
<This service is intended for profit-oriented agency only. >

Characteristics of HAC vector ;

(1) Independent chromosome

HAC vector is stably maintained as an episome.

(2) No change in DNA copy number

HAC vector behaves as same as native chromosome through cell proliferating process without change in DNA copy number.

(3) No insertion mutagenesis

HAC vector excludes unexpected insertional mutagenesis, which are involved in malignant as often happen with conventional methods.

(4) No cytotoxicity

HAC vector has no cytotoxicity observed in viral vectors, thus it is potentially effective application for gene therapy and regenerative medicine.

(5) Wide application

HAC vector can be transferred into various biological species and cell types

(6) Controllable gene expression

Expression of trangenes on HAC vector is stable, that gene expression is controllable by promoter.

(7) Large capacity

HAC vector can accommodate transgene containing a controlling region up to 300 kb of genomic DNA.

(8) Tissue-specific gene expression

Transgene on HAC vector is regulated at physiological levels of donor cells and shows tissue-specific expression.

Furthermore, we have already confirmed that HAC vector can be transferred into mouse ES cells. Transgene on HAC vector is stable and transmissible in mouse bodies, and shows tissue-specific gene expression.

If you are troubled by overexpression at transient condition of mammalian cells, or unstable expression in transformant cells, HAC vector will give you the solution of the troubles in gene transfection and cell culture, and we will propose a best application by HAC vector to fit our customers' research purpose.

■References:

  1. Ikeno M, et al., Manipulating transgenes using a chromosome vector. Nucleic Acids Res. 2009 Apr;37(6):e44. Epub 2009 Feb 17.
  2. Suzuki N, et al., Human artificial chromosomes constructed using the bottom-up strategy are stably maintained in mitosis and efficiently transmissible to progeny mice. J Biol Chem. 2006 Sep 8;281(36):26615-23
  3. Ikeno M, et al., Generation of human artificial chromosomes expressing naturally controlled guanosine triphosphate cyclohydrolase I gene. Genes Cells. 2002 Oct;7(10):1021-32.
  4. Ikeno M, et al., Construction of YAC-based mammalian artificial chromosomes. Nat Biotechnol. 1998 May;16(5):431-9.

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