Human Artificial Chromosome (HAC) is an alternative vector that promise to overcome problematic transgene expression often occurring with conventional vectors in mammalian cells and bodies.
Chromo Research provides custom service in generation of mammalian stable cell lines with HAC technology for our customers' gene and cell of interest.
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HAC vector is stably maintained as an episome.
HAC vector behaves as same as native chromosome through cell proliferating process without change in DNA copy number.
HAC vector excludes unexpected insertional mutagenesis, which are involved in malignant as often happen with conventional methods.
HAC vector has no cytotoxicity observed in viral vectors, thus it is potentially effective application for gene therapy and regenerative medicine.
HAC vector can be transferred into various biological species and cell types
Expression of trangenes on HAC vector is stable, that gene expression is controllable by promoter.
HAC vector can accommodate transgene containing a controlling region up to 300 kb of genomic DNA.
Transgene on HAC vector is regulated at physiological levels of donor cells and shows tissue-specific expression.
Furthermore, we have already confirmed that HAC vector can be transferred into mouse ES cells. Transgene on HAC vector is stable and transmissible in mouse bodies, and shows tissue-specific gene expression.
If you are troubled by overexpression at transient condition of mammalian cells, or unstable expression in transformant cells, HAC vector will give you the solution of the troubles in gene transfection and cell culture, and we will propose a best application by HAC vector to fit our customers' research purpose.
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